On super-resolved coded aperture spectral imaging software

The coded aperture snapshot spectral imaging system cassi is a remarkable optical imaging architecture, which senses the spectral information of a three dimensional scene by using two. A relatively novel technique is referred to as coded aperture snapshot spectral imaging cassi gjb07, wpsb08, wpsb09. Hamilton shepard, christy fernandezcull, ramesh raskar. In its current version, the following setups and algorithms are covered. The method uses a statistical scene model and, preferably using.

Adaptive coded aperture design by motion estimation using convolutional sparse coding in compressive spectral video sensing regular paper in. Registration and visualization of correlative super. This results in a superior excitation otf due to its lateral filling, which is rooted in the fact that the exciting and detected light is focused. Pdf coded aperture design for compressive spectral subspace. Exploiting spectral leakage for spectrogram frequency super. As such, sim is perhaps one of the most accessible superresolution techniques. The code aperture snapshot spectral imaging system cassi senses the spectral information of a scene using the underlying concepts of compressive sensing cs. We build a prototype light field projector and angleexpanding screen from scratch, evaluate the system in simulation, present a variety of results, and demonstrate that the projector can. A superresolved methodology for coded aperturebased multishot hyperspectral imaging systems has been proposed.

The 4pi concept is the result of rigorously maximizing the aperture angle employed in the imaging process. An imaging system for imaging objects, the imaging system comprising an imaging lens arrangement, a light detector unit at a certain distance. The presented approach for the super resolved imaging includes attaching a random pinhole array plate to the aperture plane of the imaging system. Cassi with a direct view, and the latest ultraviolet cassi serving a. The discovery of the diffraction barrier in 1873 by ernst abbe showed that a farfield i. Hamilton shepard, christy fernandezcull, ramesh raskar, boxin shi, 2 2 christopher barsi, and hang zhao lincoln laboratory, massachusetts institute of technology, 244 wood street, lexington, ma, 02420 mit media lab, massachusetts institute of technology, 75 amherst street, cambridge. Imaging system and method for providing extended depth of. Superresolution imaging of the extracellular space in. Infrared imaging systems design analysis modeling and.

Exploiting spectral leakage for spectrogram frequency. Compressive imaging spectrometers using coded apertures. The relay optics of the dpcassi was optimized by optical design software oslotm to minimize. Supplemental movie, appendix, image and software files for, endtoend learned, optically coded. Design, analysis, modeling, and testing xx gerald c. Osa lensfree dynamic superresolved phase imaging based on.

Us8229165b2 processing method for coded aperture sensor. Jul 26, 2006 2017 accuracy assessment and characterization of xray coded aperture coherent scatter spectral imaging for breast cancer classification. The gsted images were acquired using a leica tcs sp5 gated sted gsted microscope that was equipped with a super continuum white light laser wll and hybrid detectors adapted for. When using the mismatched time window, the spectral energy is redistributed so as to form a null. It is designed in a modular way and extendable by new algorithms in future works.

Also, the fluorescence photons may have to pass through the cell leading to aberrations and deterioration of the signal, calling for correction using adaptive optics. Wang chang, you li, zhaoyang jiao, junyong zhang, mingying sun, and jianqiang. In a snapshot, a cassi captures a twodimensional 2d array of measurements that is an. In a fluorescence microscope, diffraction prevents excitation light being focused more sharply than.

We report a novel approach to fourier ptychographic microscopy fpm by using a digital micromirror device dmd and a coherent laser source 532 nm for generating spatially modulated. Coded aperture design for superresolution phase retrieval. Alloptical super resolved and extended depth of focus. Coded aperture design for superresolution compressive x. This paper describes numerical estimation techniques for coded aperture snapshot spectral imagers cassi.

We envision that our approach, if combined with a coded aperture compressivesensing algorithm, will further improve the imaging speed in dmdbased fpm systems. An iterative regularization method for total variationbased. Brady1 1department of electrical and computer engineering, duke university, durham, nc 27708 2department of computer science, duke university, durham, nc 27708, usa 3department of electrical and computer engineering, aristotle university. In a series of papers the authors show how to construct different devices to exploit the compressive sensing paradigm crt06 which promises to enable higher resolution computational reconstructions with less samples. The energetic efficiency of the system is high and it is much larger than an imaging through a single pinhole which also has extended depth of focus. Secondly, static multiplanar coherent diffraction imaging mcdi is used toretrieve the phase of the positioned defects in small field of view. Our method uses an endtoend framework to simultaneously optimize the optical.

We established an automated registration procedure based on the generalized hough transform. The multiframe super resolution toolbox implements several stateoftheart algorithms with a common user interface. Dai dualcoded compressive hyperspectral imaging, osa optics letters journal, 39 7 compressive multimode superresolution display compressive. Image and signal processing for remote sensing, conference. The random projections in cassi are localized such that each measurement contains spectral information only from a small spatial region of the data cube. And yet the realization that a major leap in spatial resolution is possible by exploiting the spectral properties of the imaged. Using the fresnel lens as a general platform, we show three phaseplate designs, including a super thin stacked plate design, a diffractiverefractivehybrid lens, and a phase coded aperture lens. The spectral resolution is mainly constrained by the dispersion. Our method uses an endtoend framework to simultaneously optimize the optical design and a reconstruction network for obtaining super resolved images from raw measurements. A particular challenge for super resolved imaging of the tumor vasculature is the complexity of the vessel morphology with a less organized and sometimes immature vessel tree. In most imaging devices, one or more layers of optical elements are placed between the aperture and the image sensor to focus light onto the image sensor. Deepsubwavelength features of photonic skyrmions in a. Superresolution, hyperspectral imaging, compressive sensing, optical imaging, cassi, multishot, coded aperturebased systems. Snapshot hyperspectral light field imaging cvf open access.

We introduce a method for registration and visualization of correlative superresolution microscopy images from different microscopy techniques. Multispectral compressive imaging strategies using fabryp\erot. A matlab implementation of this decoding pipeline is. Video rate spectral imaging using a coded aperture. Optical design and characterization of an advanced. Endtoend learned, optically coded superresolution spad camera. The 2015 superresolution microscopy roadmap iopscience. Computational plenoptic imaging, computer graphics forum. Using the fresnel lens as a general platform, we show three phaseplate designs, including a superthin stacked plate design, a diffractiverefractivehybrid lens, and a phase codedaperture. Index termscompressive spectral imaging, coded aperture. Advanced confocal microscopy course only few places left. Compressive imaging spectrometers using coded apertures d. Digital signal processing, computational imaging, pathology, microscopy, interferometry, computer programming.

Deepsubwavelength features of photonic skyrmions in a confined electromagnetic field with orbital angular momentum luping du1, aiping yang1, anatoly v. Capturing and processing of images including occlusions. The mathematical matrix model was developed to simulate the effect of srcassi optical elements, including the decimation transformation induced by the lowresolution detector. Sep 11, 2011 in a fluorescence microscope, diffraction prevents excitation light being focused more sharply than. The samples are illuminated by a nearly coherent illumination system, where two orthogonal parallel plates are inserted into the light path and rotate to achieve controllable source microscanning. An introduction to optical superresolution microscopy for.

Dai dual coded compressive hyper spectral imaging, osa optics letters journal, 39 7 compressive multimode superresolution display compressive displays are an emerging technology exploring the codesign of new optical device configurations and compressive computation. A super resolved methodology for coded aperture based multishot hyper spectral imaging systems has been proposed. In this letter, we present a new active microscanningbased imaging platform and associated super resolution sr phase retrieval method in lensfree microscopy to achieve sr dynamic phase imaging. Moreover, multiple microscope manufacturers offer sim instrumentation, complete with easytouse image reconstruction software. Design of a tunable snapshot multispectral imaging system. The compressive measurements required by cs are obtained using a random color coded mask placed between the sensor and aperture planes.

Firstly, the defects arepositioned in large aperture by dark field imaging based on large aperture photon sieves to improve the detectionefficiency with relatively low cost. The multiframe superresolution toolbox implements several stateoftheart algorithms with a common user interface. On superresolved coded aperture spectral imaging conference paper in proceedings of spie the international society for optical engineering 8743. In this letter, we present a new active microscanningbased imaging platform and associated superresolution sr phase retrieval method in lensfree microscopy to achieve sr dynamic.

In this method, coded apertures are optimally designed based. Schematic of coded aperture snapshot spectral imaging cassi. An imaging system for imaging objects, the imaging system comprising an imaging lens arrangement, a light detector unit at a certain distance from the imaging lens arrangement, and a control unit connectable to the output of the detection unit. Pdf spatial superresolution in coded aperturebased. Siam journal on imaging sciences society for industrial. Joint coded aperture optimization and image reconstruction for compressive hyperspectral imaging. Dec 01, 2011 a relatively novel technique is referred to as coded aperture snapshot spectral imaging cassi gjb07, wpsb08, wpsb09. Highperformance computing in remote sensing, conference details. Spectral superresolution in colored coded aperture. Coded aperture imaging is a known imaging technique which is primarily used in high energy imaging such as xray or. Osa digital micromirror devicebased laserillumination. It is designed in a modular way and extendable by new algorithms in. A particular challenge for superresolved imaging of the tumor vasculature is the complexity of the vessel morphology with a less organized and sometimes immature vessel tree. Low complexity singleimage superresolution based on.

Eye, cornea, nerve, imaging systems, optical coherence tomography, image segmentation. Grady tuell, georgia tech research institute united states the world in a point cloud bernard capbern, cealaser megajoule france laser technology development and operational. Diffractionunlimited alloptical imaging and writing with a. Video rate spectral imaging using a coded aperture snapshot spectral imager ashwin a. Adding to this is the higher slice thickness in clinical imaging, which projects more intersecting tracks into a single image, and the lower frame rates, which make the. Poster session and reception institute for mathematics. This approach allows the reconstruction of superresolved hyper spectral data cubes, where the number of spectral bands is significantly increased. Spectral image estimation for coded aperture snapshot.

An iterative regularization method for total variation. Mar 04, 2019 farnoud kazemzadeh, hector lichang, alexander wong proc. The raw light fields data captured by plenotic cameras is a lenslet image from which subaperture images or views can be extracted. Spatial superresolution in code aperture spectral imaging. Phase defect detection of largeaperture optics withstatic multiplanar coherent diffraction imaging. In the proposed system, a second highresolution coded aperture is introduced in cassi to encode both spatial and spectral dimensions of the data cube. Diffractionunlimited alloptical imaging and writing with. Computational imaging with diffractive optics ubc library. Coarse estimate fine estimate superresolved estimate fig. The use of colored coded apertures in spectral compressive imaging system cassi have been shown to provide advantages in. A method of processing for a coded aperture imaging apparatus which is useful for target identification and tracking.

Multishot coded aperture snapshot spectral imaging cassi systems capture the spectral information of a scene using a small set of coded focal plane array fpa compressive measurements. We demonstrate this approach for regular intensity imaging, depth imaging, as well transient imaging. The leica dmi6000 b inverted microscope for biomedical research offers differential interference contrast dic for relief imaging of specimens with varying indices of refraction and special. Gehm duke university fitzpatrick center for photonics, durham, nc, 27708 abstract a spectral imager provides a 3d data cube in which the spatial information 2d of the image is complemented by spectral information 1d a bout each spatial location. The leica dmi6000 b inverted microscope for biomedical research offers differential interference contrast dic for relief imaging of specimens with varying indices of refraction and special diagnostics requirements. Marco bevilacqua, aline roumy, christine guillemot, and marie line alberimorel. Holst editor 1416 april 2009 orlando, florida, united states sponsored and published by spie volume. Our synthetic aperture approach enables lensfree onchip imaging to reach a numerical aperture of 1. An imaging system is presented for imaging objects within a field of view of the system. Us7646549b2 imaging system and method for providing. Computational plenoptic imaging, computer graphics forum 10.

On the other hand, imaging above the coverslip reduces the numerical aperture of the objective and thus the efficiency of collecting photons. Sted microscopy has achieved all these steps except imaging in humans and been used to visualize previously unseen details with superb resolution in many areas of life science. We report a novel approach to fourier ptychographic microscopy fpm by using a digital micromirror device dmd and a coherent laser source 532 nm for generating spatially. Fluorescence microscopy with nanometer resolution springerlink. Compressive coded aperture spectral imaging ececis. Secondly, although spatial superresolution methods have been presented 25, traditional coded aperture compressive spectral imagers usually employ coded apertures the same size as the focal.

Using an oil objective with a numerical aperture of 1. Most of our exposition is based on a professional 16camera configuration, which will be commercially available to film producers. Nov 17, 2015 in a typical imaging device, light enters through an opening aperture at one end of the imaging device and is directed to an image sensor by an optical element such as a lens. Fully automated inverted research microscope for biomedical. We developed a software tool to apply this algorithm and visualize correlated images from structured illumination microscopy sim and direct stochastic. Lowcomplexity single image superresolution based on nonnegative neighbor embedding. Superresolution imaging of the extracellular space in living. Mar 27, 2015 our synthetic aperture approach enables lensfree on chip imaging to reach a numerical aperture of 1. Highperformance computing in remote sensing, conference. Diffractive optical elements does have been studied extensively in optics for decades, but have recently received a lot of renewed interests in the context of computational imaging and computational display because they can drastically reduce. The imaging system comprises an imaging lens arrangement, a light detector unit at a certain. This approach allows the reconstruction of super resolved hyper spectral data cubes, where the number of spectral bands is significantly increased.

Highquality image restoration using lowrank patch regularization and global structure sparsity. The mathematical matrix model was developed to simulate the. Abstract pdf 2161 kb 2018 performance of the restarted homotopy perturbation method and split bregman method for. Optical design and characterization of an advanced computational imaging system 1 1 2 2,3 r. A performance evaluation of local features for imagebased 3d reconstruction. Endtoend learned, optically coded superresolution spad. In a typical imaging device, light enters through an opening aperture at one end of the imaging device and is directed to an image sensor by an optical element such as a lens. Poster session and reception institute for mathematics and. Coded aperture compressive spectral imagers allow sensing a threedimensional 3d data cube by using twodimensional 2d projections of the coded and spectrally dispersed source. Osa lensfree dynamic superresolved phase imaging based. The traditional blockunblock coded apertures have been recently replaced by patterned optical filter arrays, allowing to modulate the spatial and spectral information. Sim imaging of the actin skeleton of a hela cell beautifully demonstrate the increased resolution that can be achieved figure 26. Cannon, coded aperture imaging with uniformly redundant arrays, applied optics, vol. Spatial superresolution in coded aperture based optical.